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The application of CRISPR-based genome editing technology in plant science has opened up a new era of possibilities for improving crop traits, studying gene functions, and increasing agricultural production sustainably. To harness the full potential of CRISPR for plant genome editing, researchers need a sound understanding of how to design suitable CRISPR gene constructs. This webinar, titled "CRISPR Construct Design for Efficient Genome Editing in Plants," offers an in-depth knowledge of the critical steps involved in designing and delivering CRISPR reagents that ensure precise and effective genome editing outcomes.

The webinar will begin with an overview of how to choose the appropriate CRISPR nuclease enzyme, such as Cas9 or Cpf1, based on the specific plant gene editing and desired outcomes. Choosing the right nuclease is crucial for ensuring efficient and accurate genome editing.

The participants will learn how to select suitable target sites in the genome and design effective guide RNAs (gRNAs) for targeting specific regions. The use of various web-based software tools that simplify gRNA design, ensuring high specificity and efficiency will be explained. The webinar will also cover the design of genome editing constructs, focusing on selecting appropriate promoters to express the Cas gene and gRNA(s) in plants. Strategies for expressing multiple gRNAs and enhancing the stability of CRISPR reagents will also be discussed.

To further optimize genome editing, the participants will explore codon optimization of Cas genes and use introns to improve editing efficiency. Attendees will learn advanced techniques for designing gene constructs for multiplex CRISPR-based genome editing, allowing simultaneous editing of multiple genes. Finally, we will cover various methods for delivering CRISPR constructs into plant genomes, ranging from traditional approaches like Agrobacterium-mediated transformation to innovative techniques like nanoparticle delivery.

This webinar offers essential insights for anyone involved in plant genome editing. So, don’t miss this opportunity to enhance your skills in CRISPR technology!

This Workshop-cum-Webinar will be held online on December 4-6, 2024 with daily sessions from 5:00 pm to 8:00 pm.

Certificates will be given to all the participants.

Attendee Profile

Students, scholars, scientists and researchers from both academics and industry are encouraged to participate and take the benefit of learning details of this innovative, powerful technology from national and international experts.

The workshop-cum-webinar is ideal for plant biologists, geneticists, breeders and researchers who are engaged on applying the CRISPR technology to elucidate gene functions and enhance our understanding on various plant traits for crop improvement.

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