Agenda of 5-days Online Workshop

Welcome Address by Dr Sanjay Bajaj

Inauguration and Scope of the Workshop

Inaugural Lecture: Genome editing as a tool for basic research and crop improvement
Goetz Hensel, Head Centre for Plant Genome Engineering (CPGE), Heinrich-Heine-Universität Düsseldorf, Germany

Q/A Session

Q/A Session

End of Day 1

Advances in gene discovery and functional validation approaches in plants
Manish Pandey, Principal Scientist, ICRISAT, Hyderabad, India

Development and clinical application of CRISPR technologies
Julian Grünewald, Assistant Professor, Technical University of Munich, Germany

End of Day 2

Experimentation 1: Preparation of genome editing reagents: a) Selection of target site and in-silico designing of single-guide RNA (sgRNA) b) Use of target DNA selection program(s) c) Vector details; cloning of sgRNAs into a suitable vector d) Gene construct preparation and genetic transformation
Ajay Pandey, Scientist, National Agri-Food Biotechnology Institute (NABI), Mohali, India

Experimentation 1: Preparation of genome editing reagents: a) Selection of target site and in-silico designing of single-guide RNA (sgRNA) b) Use of target DNA selection program(s) c) Vector details; cloning of sgRNAs into a suitable vector d) Gene construct preparation and genetic transformation
Siddharth Tiwari, Scientist F, National Agri-Food Biotechnology Institute (NABI), Mohali, India

Experimentation 1: Preparation of genome editing reagents: a) Selection of target site and in-silico designing of single-guide RNA (sgRNA) b) Use of target DNA selection program(s) c) Vector details; cloning of sgRNAs into a suitable vector d) Gene construct preparation and genetic transformation
Rita Sharma, Scientist, National Agri-Food Biotechnology Institute (NABI), Mohali, India

End of Day 3

Experimentation 2: CRISPR-Cas mediated gene editing in bacterial system a) Similarities and differences between prokaryotic and eukaryotic genome editing b) CRISPR tools and techniques optimized for bacterial applications c) Experimental pipeline for CRISPRing (base editing) DNA targets in bacteria d) Case studies (studying antibiotic resistance evolution in E. coli)
Rahul Shelake, Research Professor, Gyeongsang National University, Jinju, Korea

Experimentation 3: CRISPR-Cas mediated gene editing in plants: CRISPR-Cas delivery methods: Agrobacterium, Biolistics, PEG-mediated RNPs in protoplasts Detection/analysis of edits in genome-edited plants/cell lines: - Whole genome sequencing - Sanger sequencing - T7 endonuclease 1 assay - Restriction digestion - Methodologies to obtain transgene-free mutants
Leena Tripathi, Director, International Institute of Tropical Agriculture (IITA), Kenya

End of Day 4

Experimentation 4: Case Study: Development of genome-edited rice
Inez Slamet-Loedin, Head - Rice Genetic Design & Validation Unit, IRRI, Manila, Philippines

Experimentation 5: Emerging variants of genome editing: Base editing, Prime Editing, Multiplexing, etc. and Cas systems other than Cas9, e.g., Cas 12, etc. use of AI
Kutubuddin A Molla, Scientist, ICAR-NRRI, Cuttack, India

Experimentation 5: Emerging variants of conventional genome editing: Base editing, Prime Editing, Multiplexing, etc. and Cas systems other than Cas9, e.g., Cas 12, etc. use of AI
Rahul Shelake, Research Professor, Gyeongsang National University, Jinju, Korea

Concluding Remarks- Proff. KC Bansal Workshop Director

End of Day 5