Inaugural Session

Welcome Address
Sanjay Bajaj, CEO & MD, Glostem Private Limited, India

About the Workshop by Convener
Kutubuddin A Molla, Senior Scientist, NRRI, Cuttack, India

Special Remarks by Guest of Honour
Ashwani Pareek, Executive Director, DBT - NABI, Mohali, India

Special Remarks by Guest of Honour
Viswanathan Chinnusamy, Joint Director, ICAR-IARI, New Delhi, India

Opening Remarks by Workshop Director
Prof KC Bansal, Adjunct Professor, Murdoch University, Australia

Choosing appropriate CRISPR nuclease enzyme(s)
Kutubuddin A Molla, Senior Scientist, NRRI, Cuttack, India

Selection of a suitable target site in the genome and designing an effective gRNA against the specific target : 1) gRNA design targeting a specific region in the genome 2) Use of various web-based software tools for gRNAs designing.
Kutubuddin A Molla, Senior Scientist, NRRI, Cuttack, India

Closing of Day 1

Designing genome editing constructs : 1) Selection of appropriate promoters to express the Cas gene and gRNA(s) in plants. 2) Selection of promoters for multiple gRNAs expression 3) Strategies to enhance the stability of CRISPR reagents
Sudhakar Reddy Palakolanu, Scientist, Cell, Molecular Biology & Trait Engineering, ICRISAT, Hyderabad, India

Designing gene constructs for multiplex CRISPR-based genome editing
Soham Ray, Scientist, Division of Plant Physiology, ICAR-IARI, New Delhi, India

QC of genome editing reagents and steps
Yogesh Taparia, CEO, PhytoBioworks Pvt Ltd, Bangalore, India

Closing of Day 2

Codon optimization of Cas genes and the use of intron for improving genome editing efficiency
Tom Schreiber, Dept. Cell- and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Germany

Delivery of genome editing constructs into plant cells
Satendra Kumar Mangrauthia, Senior Scientist, ICAR-IRRI, Hyderabad, India

Closing Remarks and end of the workshop
Prof KC Bansal, Adjunct Professor, Murdoch University, Australia